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Fate and transport of nitrogen (N) in urban coastal watersheds continues to draw research interest due to serious impacts of N pollution and complexities with N sources and transport pathways. In this study, we used multiple tracers for source identification of N pollution (15N isotope in nitrate and chemical sewage tracers in water) and waters (using isotopes of 18O and 2H in water) in a coastal northwest Florida U.S.A. urban bayou fed by two contrasting streams, namely Jackson Creek traversing a dense residential area and Jones Creek