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90%. The recoveries were 88%. The analytes were proven to be stable during all sample storage, preparation and analytic procedures. The method was successfully applied to determine the concentrations of nicotinamide and N1 -methylnicotinamide in human serum to investigate the association between their concentrations and obesity in 1160 Chinese subjects.Although researchers have documented behavioral and brain structure correlates of pain resilience, associated physiological responses have received little consideration. In this study,