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Purpose To describe presence of neurotrophic growth factors and histopathologic characteristics of internal limiting membrane (ILM) specimens obtained from large idiopathic full-thickness macular holes (FTMH).Methods In 24 eyes of 24 patients with FTMH of diameter 400 µm, ILM specimens were harvested directly at the edge surrounding the macular hole during vitrectomy with peeling. We performed interference and phase-contrast microscopy of flat mounts followed by immunostaining and transmission electron microscopy. Primary antigens