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© 2020 Horner et al.Aim Tacrolimus is an immunosuppressive medication with higher effectiveness compared to cyclosporine A (as a good immunosuppressant). We prepared an ophthalmic option formulation of Tacrolimus making use of hydroxypropyl beta cyclodextrin (HP-ßCD). In the present research, safety of the formulation ended up being examined in rabbits. Materials and techniques Formulation containing HP-ßCD, Tacrolimus, Polyvinyl alcohol (PVA) and Benzalkonium Chloride in PBS 7.4 was