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Little is known about how the chemical composition of enamel affects the optical properties of teeth, but advances in technology allow this to be studied using white-light and fluorescent images. This study aimed to identify the variation in enamel chemical composition that may affect tooth optical properties, such as tooth color and autofluorescence. Sixty-one specimens of extracted human molars were prepared. Raman spectrometry was used to assess chemical composition of enamel, and tooth color, and autofluorescence from enamel were e