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An overall total of 180 single-rooted real human teeth were willing to look like immature roots and split into groups (n=2. The bad control received canal irrigation just, together with positive control obtained intracanal treatment with CH for either two or twelve months. MTA blended with water had been found in Group 1 (i) without CH pre-medication - MTA(W); (ii) after 2weeks CH pre-medication - 2/52CH+MTA(W); and (iii) after 12-week CH pre-medication - 12/52 CH+MTA(W). MTA mixed with PBS
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