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Repeated intraperitoneal injection of rhRSPO1 was feasible and well tolerated. At 7 days, intestinal epithelial proliferation was increased by rhRSPO1. At 14 days, SBS+rhRSPO1 fish lost significantly less weight than SBS+PBS fish. Measurements of intestinal surface area were not increased by rhRSPO1 administration but immunofluorescent staining for β-catenin and gene expression for was increased. Intraperitoneal injection of rhRSPO1 decreased weight loss in SBS zebrafish with increased β-catenin+cells and expression at 14 days, indicati