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The reduced eruption rate and the apical shift of the contralateral incisor are probably due to an increased occlusal load. © 2020 The Authors. Anatomia, Histologia, Embryologia published by Blackwell Verlag GmbH.OBJECTIVE To investigate the influence of storage time and temperature on plasma insulin levels and to establish a correction formula. METHODS Venous blood samples were taken from 20 volunteers and processed as follows whole blood samples, centrifuged samples, and separated plasma samples were stored at 4°C or 25°C. Insulin