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001), with tympanic temperature higher at 1430 and 1730 than at 0830 and 1130. While a biological rhythm was present in tympanic temperature, pacing selection and performance when completing a 4-km cycling TT were not influenced by time of day. The findings suggest that well-trained cyclists can maintain a robust pacing strategy for a 4-km TT regardless of time of the day. While a biological rhythm was present in tympanic temperature, pacing selection and performance when completing a 4-km cycling TT were not influenced by time of day.