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Notably, both nuclear dynamics and cytoplasmic security of Frq1 and Frq2 were abolished into the lack of the FRH-coding gene nonessential for the fungal viability, highlighting an indispensability of FRH for the behaviors of Frq1 and Frq2 in both nucleus and cytoplasm. These conclusions uncover a novel circadian system harder than the well-known Neurospora design that manages rhythmic conidiation, and provide a novel understanding of molecular control over non-rhythmic conidiation in B. bassiana.Bee se