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Enzymes can aid in optimal feed stock utilization when used as feed additives. A range of toxicological studies were performed to evaluate the safety profile of a novel phytase (phytase HM) from Citrobacter b raakii produced in Aspergillus oryzae. Phytase HM was found to be non-mutagenic and non-clastogenic in in vitro tests. Further, the phytase HM preparation did not exhibit irritative potential to the eye and skin when applied in in vitro models. A 13-week subchronic toxicity study with oral administration of phytase HM to rats

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With respect, mastering and hippocampus-dependent memory had been analyzed. Male BALB/C mice received FICZ, CH223191, and XAV-939 in just one dosage of 100 μg/kg, 1 mg/kg, and 5 mg/kg of weight correspondingly via intraperitoneal (IP) shot. qRT-PCR for gene analyses and necessary protein assay in the seventh and 28th times had been performed. To assess the hippocampal centered memory, additionally they underwent contextual fear conditioning from the 28th time after tre