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Several studies have used zebrafish to investigate the effects of environmental enrichment on behavior and physiology. However, to date there are no studies evaluating the behavioral responses, such as habituation and exploration, of enriched-housed zebrafish when they are submitted to novelty paradigms. The present work was, therefore, designed to evaluate the habituation and exploratory responses of zebrafish exposed to enriched- (EE) and non-enriched (NE) environments when they face novelty. Adult wild-type zebrafish were used. Three dif