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Sixty wild waterfowl area examples had been tested and all sorts of of this four gene signals had been unambiguously identified within 6 h, such as the initial test processing in addition to final CE data analysis. The outcome indicated that multiplex RT-RPA coupled with CE had been a great alternative for instant multiple AIV detection and subtype differentiation. The large efficiency and susceptibility regarding the proposed technique could significantly help out with wild bird tracking and ep