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Male accessory gland (MAG) proteins are transferred along with the sperm to females at the time of mating and have diverse effects on female reproductive physiology in a wide range of insects. In this study, we sought to identify the MAG proteins in Leucinodes orbonalis Guenee, a Solanum melongena L. pest, by analyzing the MAG proteins of virgin and mated male moths by nano-LC-ESI-MS/MS techniques. A total of 142 and 131 proteins in virgin and mated males were identified, respectively, among which 17 (12.0%) and 10 (7.6%) proteins wer