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To investigate the influence of chemotherapy (CT) on HBsAb titer in patients receiving CT due to hematological malignancy. The data of 75 patients who received CT with the diagnosis of various hematological malignancies and who had serum HBsAb levels measured prior to and after the cessation of CT were evaluated retrospectively. The median age of the patients was 52 years (range 16-78) with 49 (65%) males and 26 (35%) females. Median HBsAb titer decreased significantly after CT compared to the pre-CT median HBsAb titer [68 (range 0-100