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Our information revealed that culture of fibroblast cells in folate- medium containing 0.5% FBS did not affect the cell cycle when compared with other groups. Flowcytometric analysis uncovered that DNA methylation (5-mC level) in folate deprived cells cultured in 0.5per cent serum had been decreased in comparison to folate+ team. The result of bisulfite sequencing was at conformity with flowcytometric analysis, which indicated a decrease in DNA methylation of POU5F1 promoter. Gene expre