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The parametrization of psb improved the fit speed 25 times (94s to 4 s). Average errors for only one fiber were 50%, 33%, and 186%, respectively. Our recently developed model provides accurate results for MDSFR measurements but not for a single fiber. The psb parametrization prevents overfitting and speeds up the fit. Our recently developed model provides accurate results for MDSFR measurements but not for a single fiber. The psb parametrization prevents overfitting and speeds up the fit. Single-molecule localization-based super-reso