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Antibacterial metal materials, including Cu- and Ag-containing alloy, have attracted much attention worldwide. As for the antibacterial mechanism of the antibacterial alloys, there are two different views metal ions sterilization and contact sterilization. For the purpose of revealing the key control factor, Titanium-silver (Ti-Ag) alloys with different silver contents were prepared and a surface acid etching was applied to change the silver ion release and the volume fraction of Ti2Ag on the surface. The microstructure, phase composit

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, transglutaminase (TGase)), and thus achieves building of centimeter-sized structure with high cell viability and technical security in vitro within 30 min. Further in vivo implantation associated with 3D-MAPS-fabricated ear-like muscle construct in rabbit designs assisted by flap prefabrication strategy outcomes in increased structural vascular support and strengthened useful success. Thus, the TGase-enhanced 3D-MAPS shows its potential and