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Genes involved in fatty acid degradation and cholesterol synthesis were high at light onset and low at night. Conclusions Our transcriptome data suggest that the highest energy demand of RPE cells is at night, whereas POS phagocytosis and degradation take place in the morning. Furthermore, we identified genes involved in fatty acid and glycerophospholipid synthesis that are upregulated at night, possibly playing a role in generating building blocks for membrane synthesis.Purpose To determine the effect of voluntary exercise on choroid