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Conclusion Fat grafts in TEC model could not only survive but also regenerate. The combination of fat graft and TEC could fabricate a vascularized fat flap, which was a promising method in breast reconstruction.Abbreviations VOI Volumes of Interest; TEC Tissue Engineering Chamber; CAL Cell Assisted Lipotransfer.Background Various graft materials and surgical methods are used in the treatment of tympanic membrane perforations.Aims/Objectives The aim of this study was to evaluate the operation method and graft success of extended pericho