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Fluoroquinolones (FQs) were frequently detected in aqueous environment. The UV irradiation have been reported as an efficient method for FQs degradation. This study investigated the influence of chemical speciation on enrofloxacin (ENR) photolysis process by UV irradiation. The results showed that chemical speciation of ENR significantly affected the photodegradation kinetics, and the highest degradation rate was observed in the zwitterion form. Presence of natural organic matter (NOM) and inorganic anions had different degrees of influen