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Trichoderma is used as a biocontrol agent against different plant pathogens in different crops. In Costa Rica, Trichoderma isolates from blackberry fruits (Rubus adenotrichos Schltdl.) have shown antagonism in laboratory and field trials against Botrytis cinerea. Quantifying fungal antagonistic activity directly on target organs or target tissues is of interest to estimate the performance of biocontrol agents. However, this is difficult due to the lack of visual manifestations of fungal structures. As part of a larger stu

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