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3% after the 3-day incubation as a result of high soil pH. The available phosphorus in soil improved significantly (p ≤ 0.01) with adding unpyrolyzed calotropis residues and its biochar produced at different pyrolysis temperatures compared to the unamended soil. The values of available phosphorus in the soil under study influenced significantly by pyrolysis temperatures of produced biochar; this is due to the pyrolysis of feedstocks increases labile phosphorus. Thenceforth, using biochar is an important strategy for enhancing carbon seq