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Shepherd's purse as a wild vegetable is getting more and more attention on health benefits. Water extract of shepherd's purse (WESP) was prepared and analyzed for the chemical constituents. The mice were fed high-fructose (HF) diet and treated with WESP at 50, 100 and 200 mg/kg·bw for 8 weeks. The HF-fed mice receiving WESP exhibited the inhibitions against abnormal weight gain, hepatic fat accumulation and lipid metabolic by down-regulating FAS and ACC expressions. WESP also significantly alleviated hyperglycemia, oxidative stress, an