https://www.selleckchem.com/pr....oducts/R788(Fostamat
SDS-PAGE and western blot analysis confirmed the successful expression of Reteplase. Western blot experiments showed most of Reteplase expressed in the insoluble form. Plasminogen cleavage assay showed significantly higher activity of the supercharged variant than the wild type protein ( 0.001). The stability of the supercharged variant was also comparable to the wild type. Our findings, i.e. the contribution of the surface supercharging technique to retained stability, enhanced plasminogen cleavage activity, wh
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