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In addition, the inhibitory mechanism of compounds a9 and b8 in HDAC16 were both compared through computational approaches, and the binding interactions between the compounds and the enzymes target were analyzed from the perspective of energy profile and conformation. In summary, the compounds with novel ZBG exhibited certain antitumor activities, providing valuable hints for the discovery of novel HDAC inhibitors. Copyright © 2020 He, Wang, Liu, Lin, Deng, Jia, Zhang, Li, Zhu, Tang, Yang, He, Jia and Zhang.Aquaporin-3 (AQP3) is