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The cartilage regeneration layer (cartilage scaffold, CS) in the BBOS includes a hyaluronic acid hydrogel to mimic the structure of cartilage, that is mechanically enhanced by host-guest supramolecular units to control the release of kartogenin (KGN). Furthermore, a 3D-printed hydroxyapatite (HAp) scaffold releasing alendronate (ALN) is utilized due to the fact bone-regeneration layer (bone tissue scaffold, BS).