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The bioleaching of chalcopyrite concentrate, intensified by the adapted mesophilic culture in the continuous stirred tank reactors (CSTR) was investigated. The cumulative bioleaching efficiency of copper was found to be increased from 34.8% to 49.3% in CSTR-1, 40.3% to 71.2% in CSTR-2, and 44.3% to 73.8% in CSTR-3, while the temperature was elevated from 30 to 37 °C, respectively; whereas, the pulp density (10%, w/v), agitation speed (350 rpm), aeration (400 cc/min), and retention time (7 days across the three reactors) were also optimi
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