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The goal of the analysis would be to gain an insight into the present molecular epidemiology of WNV in Bulgaria. STUDY DESIGN A total of 1871 Culex pipiens mosquitoes built-up in 2018 and clinical examples from 23 customers with West Nile neuroinavsive disease observed in 2018 and 2019 were tested by TaqMan RT-PCR and RT-nested PCR and PCR products had been sequenced. RESULTS WNV RNA was detected in medical samples from 10 customers as well as in five (12.2 per cent) of 41 swimming pools of
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