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The soluble GM-CSF was subjected to endotoxin removal and purification utilizing nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography, ultrafiltration. The biological task of created GM-CSF ended up being examined centered on its growth promotion impact on TF-1 cellular outlines by MTT assay technique. IU/μg was obtained.The suggested strategy right here may be used to enhance the dissolvable expression of various other hard-to-express proteins with comparable architectural pr