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The results showed that incorporation of jute fibres into the biocemented sand material had a beneficial effect, resulting in stimulation of bacterial activity, thus sustaining the MICP process during the twelve-day treatment process. This study also explores the potential of jute fibres in self-healing MICP systems.Essential genes contain key information of genomes that could be the key to a comprehensive understanding of life and evolution. Because of their importance, studies of essential genes have been considered a crucial problem in