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001, 1077±161 to 74±9 mU/dL and 8.88±0.40 to 3.79±0.56, respectively). Both parameters significantly increased with local recurrence. HE staining after denosumab treatment revealed the disappearance of giant cells and histological changes in stromal cells. Specimens of local recurrence subjected to HE staining and immunohistochemistry for H3.3 G34W demonstrated almost identical features to those in the first biopsy. Although denosumab can prevent GCTB from osteolysis, local recurrence cannot be reduced by denosumab treatment.