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Here, we report a novel vacuolar sugar transporter (ClVST1) identified through map-based cloning and connection research, whose expression in fresh fruit phloem is involving buildup of sucrose (Suc) in watermelon good fresh fruit. ClVST197 knockout lines show diminished sugar content and total biomass, whereas overexpression of ClVST197 increases Suc content. Population genomic and subcellular localization analyses strongly recommend a single-base modification at the co

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