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The results showed the presence of carcinogenic heavy metals including lead (Pb), chromium (Cr), and mercury (Hg) in the malignant colon tissues, while the healthy tissues were devoid of these elements. The concentrations of Pb, Cr and Hg in the cancerous samples were discerned to be 3.1, 13.4 and 7.1 μg/L, respectively. Furthermore, the performance accuracy of the LIBS technique was validated by comparing the results obtained from the standard inductively coupled plasma atomic emission spectroscopy (ICP-OES). It was established that t