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Three distinct techniques were employed to increase ascorbate levels in flowers (i) increased biosynthesis, (ii) enhanced recycling, or (iii) modulating regulating facets. Right here, we examine the genetic paths underlying ascorbate biosynthesis, recycling, and regulation in plants, including a listing of all metabolic engineering methods employed to time to boost ascorbate levels in model and crop species. We then highlight transgene-free strategies making use of g
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