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This study aimed to determine the median lethal concentration (96-h LC5, acute and sublethal effects of malathion, an organophosphorus pesticide on hematological and biochemical responses in an Indian major carp, Labeo rohita. In this study, the LC50 value of malathion for 96 h was found to be 3.4 ppm. During acute (3.4 ppm) and sublethal [1/10th of 96 h LC50 value (0.34 ppm) studies, all the hematological parameters except WBC were significantly decreased (p less then 0.05). Besides, when compared with the control group, a