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Concentrations of copper (Cu), iron (Fe), manganese (Mn), nickel (Ni) and zinc (Zn) in muscle and gills of four Benthic fish species (Brachirus orientalis, Argyrops spinifer, Platycephalus indicus and Sillago sihama) from Shif Island of Iran were analyzed using flame atomic absorption spectrometry. The heavy metal levels observed in tissues varied for Cu 2.15-820.98, Fe 24.46-533.26, Mn 1.80-53.17, Ni 1.86-65.54 and Zn 28.77-375.79 mg/kg. Significant differences were found among muscle and gill of the four fish species. Heavy metals con