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The inhibition diameter of strain PJ_11 ranged from 17.63 to 26.68 mm. In co-culture assay with the strain PJ_11 culture, the growth of V. vulnificus, V. parahaemolyticus and V. anguillarum was inhibited after 120 h with an initial level of 1.0 × 108 CFU/mL. PJ_11 had antibiotic susceptibility to commonly used antibiotics in aquaculture but only resistant to chloramphenicol. Isolate was non-hemolytic. These findings suggested that the strain PJ_11 is good probiotic candidate and may have potential applications to prevent/control vibriosi