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The dsRNA phrase efficiency of our book E. coil system was 3 times that of L4440-HT115(DE3), a widely used dsRNA production system. The RNAi effectiveness of dsRNA produced by our system and also by biochemical synthesis had been similar whenever inserted into Harmonia axyridis. SUMMARY Our system expressed dsRNA more efficiently than the trusted L4440-HT115(DE3) system, and the produced dsRNA revealed a higher gene-silencing effect. Notably, our pET28-BL21(DE3) RNase III-system provides a n
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