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Sub-tomogram averaging reveals that the ESCRT-III filaments assemble parallel and locally perpendicular towards the tube axis, highlighting the mechanical stresses enforced by ESCRT-III. Our results underline the versatile membrane layer renovating task of ESCRT-IIwe that could be an over-all function necessary for mobile membrane renovating processes.Increasing evidence indicates that assistance molecules utilized during development for cellular and axonal navigation also play functi