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Both chemotaxis and biofilm development perform a crucial role in nitrogen-fixing bacteria and their discussion making use of their host flowers. Interestingly, AZC_2928 would not affect the motility of A. caulinodans ORS571 and the nodulation development in their all-natural host plant, Sesbania rostrata. Due to rhizobia having to form bacteroids for symbiotic nitrogen fixation in mature nodules, AZC_2928 might have a direct impact on nitrogen fixation effect