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More specifically, an engineered bacterial vector carrying the LyE gene showed improved gene delivery and safety profiles when tested with murine RAW264.7 macrophage APCs. This chapter outlines steps taken to engineer E. coli for LyE expression as a safer and more effective genetic antigen delivery bactofection vehicle in the context of vaccine utility. Prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT) with Lu-labeled PSMA ligands has achieved remarkable results in advanced disease stages of metastatic castrati