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Spiders produce a diversity of silk fibers from multiple morphologically distinct silk glands for specific tasks, and each silk type primarily composed of one or more particular silk proteins encoded by silk gene family members believed to generated by duplication and divergence of ancient silk genes. Egg case silks spun from tubuliform glands are used to construct the tough outer structure of egg cases, are important for their reproduction. Here we present two novel complete TuSp1 sequences from orb weaving spider Araneus ventricosus

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