https://www.selleckchem.com/pr....oducts/pf-06650833.h
Following callus formation and de novo shoot regeneration, the regenerated inflorescence stems were used for de novo root organogenesis. The entire protoplast regeneration process was completed within 15weeks. The in vitro regenerated plants were fertile and produced morphologically normal progenies. The cell and tissue culture system optimized in this study for protoplast regeneration is efficient and reproducible. This method of Arabidopsis protoplast regeneration can be used for fundamental studies on pluripotency establishment a
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