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To examine the association of various gender-affirming hormone therapy regimens with blood sex hormone concentrations in transgender individuals. This retrospective study included transgender people receiving gender-affirming hormone therapy between January 2000 and September 2018. Data on patient demographics, laboratory values, and hormone dose and frequency were collected. Nonparametric tests and linear regression analyses were used to identify factors associated with serum hormone concentrations. Overall, 196 subjects (134 transgend