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After four rounds of backcrossing to Optic, plants were genotyped and their progeny were tested for transformability. Some of the progeny lines were transformable at high efficiencies similar to those seen for the parent M1460 and some were not transformable, like Optic. A region on chromosome 2H inherited from M1460 is present in transformable lines only. We propose that one of the 225 genes in this region is TRA1. Copyright © 2020 Orman-Ligeza, Harwood, Hedley, Hinchcliffe, Macaulay, Uauy and Trafford.A pyruvate-phosphate dikinase (C4