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Perinatal Pb treatment was associated with 12.4% more oligodendrocytes (P=4.4x10-21) in adult mice. Across all cells, Pb treatment was associated with expression of cell cluster marker genes. Within cell clusters, Pb treatment (q less then 0.05) caused differential gene expression in endothelial, microglial, pericyte, and astrocyte cells. Pb treatment up-regulated protein folding pathways in microglia (P=3.4x10-9) and stress response in oligodendrocytes (P=3.2x10-5). Conclusion Bulk tissue analysis may be influenced by changes in cell ty