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Although pcDNA- and pVAX- both elicited protective immunity, mice immunized with the latter showed a higher protection against both and PA76250. The results of this study highlight the significant differences between efficiency of diverse plasmid backbones in DNA vaccines which code for an identical antigen. Comparing various plasmid vectors should be considered as an essential part of the studies aiming construction of DNA vaccines for intracellular pathogens. The results of this study highlight the significant differences between effi