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The elementary product of PT is a PT pore (PTP), an individual channel apparently created by either ATP synthase or adenine nucleotide translocator (ANT). It is not understood what amount of stations are available in a single mitochondrion during PT, which makes it difficult to quantitatively estimate the overall amount of membrane layer permeability. Here, we utilized wide-field microscopy to record mitochondrial swelling and quantitatively measure rates of single