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Methylobacterium spp. are emerging opportunistic premise plumbing pathogens. Human infections linked to premise plumbing provide evidence of their routes of infection. Cells of a collection of representative strains of different Methylobacterium species were tested for hydrophobicity by contact angle, adherence and biofilm formation on different plumbing materials, and temperature tolerance (50-60 °C); characteristics shared by OPPPs. Methylobacterium spp. strains were shown to grow in drinking water, have high cell-surface hydrophobic